Kapa Biosystems官網 kapa試劑中國代理商


Kapa Biosystems官網 kapa試劑中國代理商

Kapa是世界上著名的生物技術公司。總部位于美國波士頓, 公司的主要理念是運用分子和遺傳學技術來打造新一代的生物科學和醫學診斷產品。
Kapa公司目前主要是在“直接進化(directed evolution technology platform)”的技術平臺上來篩選和生產高品質的生物酶。目前生物醫藥領域所使用的大部分生物酶都只是天然存在形式,即使有些酶經過基因工程改造,但是由于酶分子自身的結構和功能限制,要想得到真正滿足科研工作人員需要的產品,卻是一件費時費力的事情,而且常常達不到預期的要求。“直接進化”技術正是針對這一現實情況,運用遺傳,基因工程,生物信息學等技術來在分子水平上設計真正滿足不同實際要求的生物酶。通過創新,Kapa為廣大的科研人員提供了一系列擁有多項技術的產品,不僅能夠滿足普通科研需要,同時為高通量,基因芯片等高新技術的普及創造了條件。 運用“直接進化”技術,Kapa目前已經開發出來了以下的高品質產品:
1. Kapa2G超強PCR (Kapa2G Robust PCR)。適用范圍:可用于普通的PCR反應中;AT和GC堿基含量高的擴增;模板 中含有常見的PCR反應抑制物,例如,鹽離子,尿素,SDS,乙醇,EDTA等;石蠟油包被樣品;糞便樣品;菌落PCR。
2. Kapa2G 快速PCR(Kapa2G Fast PCR)。高靈敏度,高特異性。延伸速度高達1秒/kb,(常規延伸速度是60s/kb),可擴增出長達5kb的目的片段,節省大約75%的反應時間。
3.實時熒光定量PCR (Kapa SYBR qPCR)。和其他公司的類似產品相比,Kapa SYBR qPCR改造了Taq DNA多聚酶,而不是對熒光染料進行化學改造,因此大大提高了信號強度以及反應的有效性。更重要的是,Kapa的系列產品可以適用于市場上現有的Real-time PCR儀器。
4.Kapa長片段DNA多聚酶 (Kapa LongRange)。Kapa長片段DNA多聚酶是由Taq DNA聚合酶和修飾過的archael(B型)高保真DNA聚合酶組成的雙酶系統。后者含有校正功能。這種雙酶系統是專門為長片段和敏感PCR擴增而設計的。一般而言,普通的Taq酶由于缺少校正功能,因此隨著錯誤堿基的不斷產生,使得擴增過早的終止,從而大大降低了擴增效率。這也是導致在擴增長片段或者模板濃度很低時,使用普通Taq酶常常失敗的原因。另一方面,雖然具有校正能力的多聚酶擁有很高的正確率,但由于其3到5的外切酶活性,引物會被降解掉,從而也影響到擴增的靈敏度。Kapa長片段產品的原理是:在Taq DNA多聚酶中加入具有校正能力并且修飾過的多聚酶,可以使得不匹配的堿基被修正;同時,和只有校正功能的多聚酶相比,kapa的雙酶系統中具有校正功能的酶所占的比例較少,因此可以相對延長擴增的循環數和時間,從而提高產量,同時得到高保真的PCR產物。雖然兩種酶都有5到3的DNA聚合功能,但是只有Taq酶具有依賴于雙鏈的5到3的聚合酶活性,而只有B型DNA聚合酶具有3到5的外切酶功能(也就是校正功能)。
5.Kapa高保真DNA多聚酶 (Kapa Fidelity)。是經過修飾的,耐熱的高保真DNA多聚酶。既有5到3的多聚酶活性,又有3到5外切校正功能,因此能夠擴增出優質的高保真片段。高保真:Kapa高保真DNA多聚酶的擴增出正確片段的幾率是普通Taq酶的15倍,KOD/Pfx2倍;應用廣泛:可以擴增出長至6kb的質粒或者lambda DNA,或者長至2kb的基因組DNA擴增;用量少:由于其高活性,和其它的高保真產品相比,每個反應的用量要少,從而為實驗者節省資金;易于克隆:Kapa高保真DNA多聚酶擴增出鈍頭產物,非常易于克隆;快速:擴增速度是pfu的2-3倍。 另外,還有普通的KapaTaq DNA多聚酶,以及熱啟動酶,T4連接酶,快速連接試劑盒等產品。

 

部分產品報價:

第二代基因工程酶及相關產品
產品歸類 廠家 貨號 品名 規格 目錄價
HiFi高保真酶 KAPA KK2101 HiFi 100 units 700
KAPA KK2102 HiFi 250 units 1600
KAPA KK2501 HiFi Hot Start 100 units 800
KAPA KK2502 HiFi Hot Start 250 units 1800
KAPA KK2601 HiFi Hot Start 100 rxn 800
KAPA KK2602 HiFi Hot Start 500 rxn 3500
HiFi高保真酶(二代測序專用) KAPA KK2611 HiFi Hot Start for NGS 50 rxn 800
KAPA KK2612 HiFi Hot Start for NGS 250 rxn 3500
KAPA KK2701 HiFi Hot Start for NGS 50 rxn 2400
KAPA KK2702 HiFi Hot Start for NGS 250 rxn 10000
KAPA KK2801 KAPA HiFi? HS Uracil +ready Mix 50 x 50μL 3000
KAPA KK2802 KAPA HiFi? HS Uracil +ready Mix 250 x 50μL 14000
長片段酶 KAPA KK3006 Long Range 100 units 800
KAPA KK3005 Long Range 250 units 1600
KAPA KK3501 Long Range Hot Start 100 units 900
KAPA KK3502 Long Range Hot Start 250 units 1800
KAPA KK3503 Long Range Hot Start 500 units 3000
KAPA KK3601 Long Range Hot StartReady Mix with DYE ?100 x 25μL 1100
KAPA KK3602 Long Range Hot StartReady Mix with DYE ?500 x 25μL 4500
與takara酶相對應的KAPA Extaq酶 KAPA KK3009 KAPATaq Extra(針對takara ExTaq) 250units 400
KAPA KK3505 KAPATaq Extra Hot Start(針對takara ExTaq) 250units 520
KAPA KK3604 KAPATaq Extra Hot Start Ready Mix with DYE (針對takara ExTaq) ?100 x 25μL 400
KAPA KK3605 KAPATaq Extra Hot StartReady Mix with DYE(針對takara ExTaq) ?500 x 25μL 1800
KAPA KK3606 KAPATaq Extra Hot Start Ready Mix with DYE ?200 x 25μL 800
HRM KAPA KK4201 KAPA HRM FAST 1ml 700
KAPA KK4202 KAPA HRM FAST 5ml 3200
KAPA KK4203 KAPA HRM FAST 10ml 6000
SYBR熒光定量 KAPA KK4600 SYBR? Fast通用型 1 ml 400
KAPA KK4601 SYBR? Fast通用型 5 ml 1800
KAPA KK4602 SYBR? Fast通用型 10 ml 3500
KAPA KK4618 SYBR? Fast通用型 50 ml 17000
KAPA KK4603 SYBR? Fast(ABI Prism) 1 ml 400
KAPA KK4604 SYBR? Fast(ABI Prism) 5 ml 1800
KAPA KK4605 SYBR? Fast(ABI Prism) 10 ml 3500
KAPA KK4617 SYBR? Fast(ABI Prism) 50ml 17000
KAPA KK4620 SYBR FAST ROX Low 500*20ul 1800
KAPA KK4606 SYBR? Fast(伯樂專用型) 1 ml 400
KAPA KK4607 SYBR? Fast(伯樂專用型) 5 ml 1800
KAPA KK4608 SYBR? Fast(伯樂專用型) 10 ml 3500
KAPA KK4609 SYBR? Fast (Roche LC 480) 1 ml 400
KAPA KK4610 SYBR? Fast(Roche LC 480) 5 ml 1800
KAPA KK4611 SYBR? Fast(Roche LC 480) 10 ml 3500
SYBR 一步法Qpcr KAPA KK4650 1-step qRT-PCR Kit(通用型) 1ml 1000
KAPA KK4651 1-step qRT-PCR Kit(通用型) 5ml 4500
KAPA KK4652 1-step qRT-PCR Kit(通用型) 10ml 8000
KAPA KK4660 1-step qRT-PCR Kit(ABI Prism) 1ml 1000
KAPA KK4661 1-step qRT-PCR Kit(ABI Prism) 5ml 4500
KAPA KK4662 1-step qRT-PCR Kit(ABI Prism) 10ml 8000
KAPA KK4670 1-step qRT-PCR Kit(伯樂專用型) 1ml 1000
KAPA KK4671 1-step qRT-PCR Kit(伯樂專用型) 5ml 4500
KAPA KK4672 1-step qRT-PCR Kit(伯樂專用型) 10ml 8000
KAPA KK4680 1-step qRT-PCR Kit(Roche LC 480) 1ml 1000
KAPA KK4681 1-step qRT-PCR Kit(Roche LC 480) 5ml 4500
KAPA KK4682 1-step qRT-PCR Kit(Roche LC 480) 10ml 8000
PROBE熒光定量 KAPA KK4701 PROBE FAST(通用型) 1 ml 400
KAPA KK4702 PROBE FAST(通用型) 5 ml 1800
KAPA KK4703 PROBE FAST(通用型) 10 ml 3500
KAPA KK4705 PROBE FAST(ABI Prism) 1 ml 400
KAPA KK4706 PROBE FAST(ABI Prism) 5 ml 1800
KAPA KK4707 PROBE FAST(ABI Prism) 10 ml 3500
KAPA KK4709 PROBE FAST(伯樂專用型) 1 ml 400
KAPA KK4710 PROBE FAST(伯樂專用型) 5 ml 1800
KAPA KK4711 PROBE FAST(伯樂專用型) 10 ml 3500
KAPA KK4714 PROBE FAST(ABI Prism,定制50ml體系) 50ml 17000
KAPA KK4715 PROBE FAST(通用型,定制50ml體系) 50ml 17000
KAPA KK4716 PROBE FAST (ROX Low Mastermix) 1ml 400
KAPA KK4717 PROBE FAST(ROX Low Mastermix) 5 ml 1800
KAPA KK4718 PROBE FAST(ROX Low Mastermix) 10 ml 3500
KAPA KK4719 PROBE FAST(ROX Low Mastermix) 50 ml 17000
KAPA KK4722 PROBE FAST Univrsal qPCR Kit with dUTP 500x20ul 1800
PROBE Force qPCR KAPA KK4752 PROBE FAST(1-step qRT-PCR Probe Universal) 5 ml 4500
KAPA KK4300 通用型PROBE FORCE定量試劑 1ml 700
KAPA KK4301 通用型PROBE FORCE定量試劑 5ml 3500
KAPA KK4302 通用型PROBE FORCE定量試劑 10ml 6000
KAPA KK4303 通用型PROBE FORCE定量試劑 50ml 26000
2G Robust酶及相關產品 KAPA KK5023 ?2G Robust 100 units 400
KAPA KK5024 ?2G Robust 250 units 900
KAPA KK5004 ?2G Robust(含dNTPs) 100units 400
KAPA KK5005 ?2G Robust(含dNTPs) 250 units 900
KAPA kk5522 ?2G Robust Hot Start 100units 600
KAPA KK5515 ?2G Robust Hot Start 250 units 1400
KAPA KK5517 ?2G Robust Hot Start 500 units 2400
KAPA KK5525 ?2G Robust Hot Start 2500units 9000
KAPA KK5532 ?2G Robust Hot Start(含dNTPs) 100 units 700
KAPA KK5516 ?2G Robust Hot Start(含dNTPs) 250 units 1500
KAPA KK5518 ?2G Robust Hot Start(含dNTPs) 500units 2800
KAPA KK5701 ?2G Robust Hot Start Readymix 100rxn 700
KAPA KK5702 ?2G Robust Hot Start Readymix 500 rxn 2600
2G Fast酶及相關產品 KAPA KK5523 ?2G Fast Hot Start 100 units 600
KAPA KK5503 ?2G Fast Hot Start 250 units 1300
KAPA KK5501 ?2G Fast Hot Start 500 units 2400
KAPA KK5519 ?2G Fast Hot Start 2500 units 7000
KAPA KK5530 ?2G Fast Hot Start(含dNTPs) 100 units 700
KAPA KK5502 ?2G Fast Hot Start(含dNTPs) 250 units 1500
KAPA KK5500 ?2G Fast Hot Start(含dNTPs) 500 units 2600
KAPA KK5513 ?2G Fast Hot Start(Buffer無Mg2+) 250 units 1400
KAPA KK5511 ?2G Fast Hot Start(Buffer無Mg2+) 500 units 2400
KAPA kk5512 ?2G Fast Hot Start(含dNTPs,Buffer無Mg2+) 250 units 1500
KAPA KK5510 ?2G Fast Hot Start(含dNTPs,Buffer無Mg2+) 500 units 2800
KAPA KK5603 ?2G Fast Hot Start ReadyMix 100 rxn 400
KAPA KK5601 ?2G Fast Hot Start ReadyMix 500 rxn 1400
KAPA KK5801 ?2G Fast Hot Start 100 rxn 500
KAPA KK5802 ?2G Fast Hot Start 500 rxn 1900

 

二代測序相關產品
廠家 貨號 產品名稱 規格 目錄價
二代測序文庫制備 KAPA KK8230 KAPA LTP文庫構建(含文庫擴增)試劑盒-Illumina 10個文庫 2500
KAPA KK8231 KAPA LTP文庫構建(不含文庫擴增)試劑盒-Illumina 10個文庫 2400
KAPA KK8232 KAPA LTP文庫構建(含文庫擴增)試劑盒-Illumina 50個文庫 11000
KAPA KK8233 KAPA LTP文庫構建(不含文庫擴增)試劑盒-Illumina 50個文庫 10500
KAPA KK8234 KAPA HTP文庫構建(含文庫擴增)試劑盒-Illumina 96個文庫 18000
KAPA KK8235 KAPA HTP文庫構建(不含文庫擴增)試劑盒-Illumina 96個文庫 17000
KAPA KK8260 KAPA TruSeq Adapter Kit, (Illumina, 20 μl each) 144 rxn 14000
KAPA KK2620 KAPA Library Amplification Kit with Primers (50 x 50 μl reactions) 50rxn 1800
KAPA KK2621 KAPA Library Amplification Kit with Primers (250 x 50 μl reactions) 250rxn 8000
KAPA KK2623 KAPA Library Amplification Primer Kit (250 x 50 μl rxns) 250rxn 1500
KAPA KK8400 KAPA Stranded RNA-Seq Library Preparation Kit (Illumina,24 reactions) 24 rxn 13000
KAPA KK8401 KAPA Stranded RNA-Seq Library Preparation Kit (96 reactions) 96 rxn 40000
KAPA KK8420 KAPA Stranded mRNA-Seq Library Preparation Kit (24 reactions) 24 rxn 15000
KAPA KK8421 KAPA Stranded mRNA-Seq Library Preparation Kit (96 reactions) 96 rxn 47000
KAPA KK8483 KAPA Stranded RNA-Seq Kit with RiboErase(HMR) (24rxns) 24rxn 28000
KAPA KK8484 KAPA Stranded RNA-Seq Kit with RiboErase(HMR) (96rxns) 96rxn 98000
KAPA KK8500 KAPA Hyper Prep Kit (8 reactions) 8 rxn 3100
KAPA KK8501 KAPA Hyper Prep Kit, no amplification (8 reactions) 8 rxn 2970
KAPA KK8502 KAPA Hyper Prep Kit (24 reactions) 24 rxn 8100
KAPA KK8503 KAPA Hyper Prep Kit, no amplification (24 reactions) 24 rxn 7830
KAPA KK8504 KAPA Hyper Prep Kit (96 reactions) 96 rxn 28100
KAPA KK8505 KAPA Hyper Prep Kit, no amplification (96 reactions) 96 rxn 27000
KAPA KK8510 KAPA Hyper Plus Kit (Illumina,8 rxns) 8 rxn 3500
KAPA KK8511 KAPA Hyper Plus Kit, PCR-free (Illumina,8 rxns) 8 rxn 3370
KAPA KK8512 KAPA Hyper Plus Kit (Illumina,24 rxns) 24 rxn 9300
KAPA KK8513 KAPA Hyper Plus Kit, PCR-free (Illumina,24rxns) 24 rxn 9030
KAPA KK8514 KAPA Hyper Plus Kit (Illumina,96 rxns) 96 rxn 32900
KAPA KK8515 KAPA Hyper Plus Kit, PCR-free (Illumina,96 rxns) 96 rxn 31800
KAPA KK8300 Ion Torrent? Library Preparation Kit (8 reactions) 8 rxn 2520
KAPA KK8301 Ion Torrent?? Library Preparation Kit (48 reactions) 48 rxn 13860
KAPA KK8310 Ion Torrent Library Preparation Kit, no amplification (8 reactions) 8 rxn 2280
KAPA KK8311 Ion TorrentLibrary Preparation Kit, no amplification (48 reactions) 48 rxn 12420
KAPA KK8330 Adapter Kit, non-Barcoded (8 reactions??? Ion Torrent 8 rxn 1200
KAPA KK8331 Adapter Kit, Barcodes 1 – 8 (48 reactions)?? Ion Torrent 48 rxn 7500
KAPA KK8332 Adapter Kit, Barcodes 9 – 16 (48 reactions)?? Ion Torrent 48 rxn 7500
KAPA KK8333 Adapter Kit, Barcodes 17 – 24 (48 reactions)?? Ion Torrent 48 rxn 7500
二代測序文庫定量:標準品+引物預混物 KAPA KK4800 DNA Quant Kit?? Roche 454 FLX Each 6000
KAPA KK4801 Primer Premix? Roche 454 FLX Each 3000
KAPA KK4802 DNA Quant Kit? Roche 454 Titanium Each 6000
KAPA KK4803 Primer Premix Roche 454 Titanium Each 3000
KAPA KK4804 DNA Quant Kit? Illumina Genome Analyzer Each 6000
KAPA KK4805 Primer Premix? Illumina Genome Analyzer Each 3000
KAPA KK4806 DNA Quant Kit? ABI SOLiD Each 6000
KAPA KK4807 Primer Premix? ABI SOLiD Each 3000
KAPA KK4808 DNA Quant Kit?? Illumina GA Revised Primers Each 6000
KAPA KK4809 Primer Premix? Illumina GA Revised Primers Each 3000
KAPA KK4812 DNA Quant Kit – Ion Torrent Each 6000
KAPA KK4813 DNA Quantification Primer Premix –? Ion Torrent Each 3000
KAPA KK4900 Library Quantification DNA Standards (454 FLX/Lib-A) 6 x 80 μl 4000
KAPA KK4902 Library Quantification DNA Standards (SOLiD) 6 x 80 μl 4000
KAPA KK4903 Library Quantification DNA Standards (Illumina) 6 x 80 μl 4000
KAPA KK4904 Library Quantification DNA Standards (Ion Torrent) 6 x 80 μl 4000
KAPA KK4923 KK4923Library Quant Primers+KSF(IIIumina/Uni) IIIumina/Uni 4000
KAPA KK4924 kk4924 Library Quant primers+KSF(10mTorrent/uni) 10mTorrent/uni 5000
KAPA KK4820 Library? Quant Kit,Roche 454 FLX-SYBR Fast Universal Each 7000
KAPA KK4821 Library? Quant Kit,Roche 454 Titanium-SYBR Fast Universal Each 7000
KAPA KK4824 Library? Quant Kit,Illumina GA revised primers -SYBR Fast Universal Each 7000
KAPA KK4823 Library? Quant Kit,ABI SOLiD-SYBR Fast Universal Each 7000
KAPA KK4830 Library? Quant Kit,Roche 454 FLX-SYBR Fast ABI Prism Each 7000
KAPA KK4831 Library? Quant Kit,Roche 454 Titanium-SYBR Fast ABI Prism Each 7000
KAPA KK4835 Library? Quant Kit,Illumina GA revised primers -SYBR Fast ABI Prism Each 7000
KAPA KK4833 Library? Quant Kit,ABI SOLiD-SYBR Fast ABI Prism Each 7000
KAPA KK4840 Library? Quant Kit,Roche 454 FLX-SYBR Fast Bio-Rad iCycler Each 7000
KAPA KK4841 Library? Quant Kit,Roche 454 Titanium-SYBR Fast Bio-Rad iCycler Each 7000
KAPA KK4844 Library? Quant Kit,Illumina GA revised primers-SYBR Fast Bio-Rad iCycler Each 7000
KAPA KK4843 Library? Quant Kit,ABI SOLiD-SYBR Fast Bio-Rad iCycler Each 7000
KAPA KK4850 Library? Quant Kit,Roche 454 FLX-SYBR Fast Roche Light Cycler480 Each 7000
KAPA KK4851 Library? Quant Kit,Roche 454 Titanium-SYBR Fast Roche Light Cycler 480 Each 7000
KAPA KK4854 Library? Quant Kit,Illumina GA revised primers-SYBR Fast Roche Light Cycler480 Each 7000
KAPA KK4853 Library? Quant Kit,ABI SOLiD-SYBR Fast Roche Light Cycler480 Each 7000
KAPA KK4834 Library? Quant Kit,Illumina GA ABI Prism Custom Kit- Sanger Each 7000
KAPA KK4827 Library? Quant Kit,Ion Torrent PGM universal Each 7000
KAPA KK4838 Library? Quant Kit,Ion Torrent PGM ABI Prism Each 7000
KAPA KK4847 Library? Quant Kit,Ion Torrent PGM? Bio-Rad iCycler Each 7000
KAPA KK4857 Library? Quant Kit,Ion Torrent PGM? Roche Light Cycler480 Each 7000
KAPA KK4873 KK4873Library Quant Kit (Illumina/ROX Low) 500rxn 7000
人類基因組專用定量Kit(文庫構建之前用,主要檢測提取的DNA量) KAPA KK4960 hg DNA Quant & QC kit with SYBR Universal qPCR Mastermix Each 8000
KAPA KK4961 hg DNA Quant & QC kit with SYBR ABI Prism qPCR Mastermix Each 8000
KAPA KK4962 hg DNA Quant & QC kit with SYBR Bio-Rad qPCR Mastermix Each 8000
KAPA KK4963 hg DNA Quant & QC kit with SYBR Light Cycler 480 qPCR Mastermix Each 8000
KAPA KK4964 hg DNA Quant & QC DNA Standards Kit Each 6000
KAPA KK4965 hg DNA Quant & QC and 41 bp Primer Kit Each 1500
KAPA KK4966 hg DNA Quant & QC and 129 bp Primer Kit Each 1500
KAPA KK4967 hg DNA Quant & QC and 305 bp Primer Kit Each 1500
KAPA KK4969 hg DNA Quant & QC Kit with ROX Low qPCR Mastermix Each 8000